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甘丙肽樣肽（GALP）是一種60氨基酸的神經(jīng)肽，與甘丙肽不同，它具有非酰胺化的C端。
編號：146875
CAS號：245114-97-0
單字母：H2N-APAHRGRGGWTLNSAGYLLGPVLHLSSKANQGRKTDSALEILDLWKAIDGLPYSRSPRMT-OH
Galanin-like peptide (GALP), first isolated from the porcine hypothalamus and subsequently also identified in rats and humans, is a 60 amino acid neuropeptide that unlike galanin, has a non-amidated C-terminus. Its amino acid residues (9-21) are identical to the biologically active N-terminal (1-13) fragment of galanin. In contrast to galanin which is relatively non-selective, receptor binding studies revealed that GALP had a high affinity for the galanin receptor 2 (GALR2) and a lower affinity for the GALR1 receptor. Furthermore, it could be demonstrated that GALP stimulates food intake with a ten fold higher orexigenic activity than galanin and may also affect emotional state in the CNS.
| DOI | 名稱 | |
|---|---|---|
| 10.1016/s0304-3940(01)02515-0 | Galanin-like peptide stimulates food intake in the rat | 下載 |
| 10.1007/s12031-011-9546-y | Distribution of alarin immunoreactivity in the mouse brain | 下載 |
多肽H2N-Ala-Pro-Ala-His-Arg-Gly-Arg-Gly-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Val-Leu-His-Leu-Ser-Ser-Lys-Ala-Asn-Gln-Gly-Arg-Lys-Thr-Asp-Ser-Ala-Leu-Glu-Ile-Leu-Asp-Leu-Trp-Lys-Ala-Ile-Asp-Gly-Leu-Pro-Tyr-Ser-Arg-Ser-Pro-Arg-Met-Thr-COOH的合成步驟：
1、合成CTC樹脂：稱取1.16g CTC Resin（如初始取代度約為1.15mmol/g）和1.6mmol Fmoc-Thr(tBu)-OH于反應(yīng)器中，加入適量DCM溶解氨基酸（需要注意，此時CTC樹脂體積會增大好幾倍，避免DCM溶液過少），再加入4.0mmol DIPEA（Mw：129.1,d：0.740g/ml），反應(yīng)2-3小時后，可不抽濾溶液，直接加入1ml的HPLC級甲醇，封端半小時。依次用DMF洗滌2次，甲醇洗滌1次，DCM洗滌一次，甲醇洗滌一次，DCM洗滌一次，DMF洗滌2次（這里使用甲醇和DCM交替洗滌，是為了更好地去除其他溶質(zhì)，有利于后續(xù)反應(yīng)）。得到 Fmoc-Thr(tBu)-CTC Resin。結(jié)構(gòu)圖如下：
2、脫Fmoc：加3倍樹脂體積的20%Pip/DMF溶液，鼓氮氣30分鐘，然后2倍樹脂體積的DMF 洗滌5次。得到 H2N-Thr(tBu)-CTC Resin 。（此步驟脫除Fmoc基團，茚三酮檢測為藍(lán)色，Pip為哌啶）。結(jié)構(gòu)圖如下：
3、縮合：取4.0mmol Fmoc-Met-OH 氨基酸，加入到上述樹脂里，加適當(dāng)DMF溶解氨基酸，再依次加入8.0mmol DIPEA，3.8mmol HBTU。反應(yīng)30分鐘后，取小樣洗滌，茚三酮檢測為無色。用2倍樹脂體積的DMF 洗滌3次樹脂。（洗滌樹脂，去掉殘留溶劑，為下一步反應(yīng)做準(zhǔn)備）。得到Fmoc-Met-Thr(tBu)-CTC Resin。氨基酸：DIPEA：HBTU：樹脂=3：6：2.85：1（摩爾比）。結(jié)構(gòu)圖如下：
4、依次循環(huán)步驟二、步驟三，依次得到
H2N-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
以上中間結(jié)構(gòu)，均可在專肽生物多肽計算器-多肽結(jié)構(gòu)計算器中，一鍵畫出。
最后再經(jīng)過步驟二得到 H2N-Ala-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin，結(jié)構(gòu)如下：
5、切割：6倍樹脂體積的切割液（或每1g樹脂加8ml左右的切割液），搖床搖晃 2小時，過濾掉樹脂，用冰無水乙醚沉淀濾液，并用冰無水乙醚洗滌沉淀物3次，最后將沉淀物放真空干燥釜中，常溫干燥24小試，得到粗品H2N-Ala-Pro-Ala-His-Arg-Gly-Arg-Gly-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Val-Leu-His-Leu-Ser-Ser-Lys-Ala-Asn-Gln-Gly-Arg-Lys-Thr-Asp-Ser-Ala-Leu-Glu-Ile-Leu-Asp-Leu-Trp-Lys-Ala-Ile-Asp-Gly-Leu-Pro-Tyr-Ser-Arg-Ser-Pro-Arg-Met-Thr-COOH。結(jié)構(gòu)圖見產(chǎn)品結(jié)構(gòu)圖。
切割液選擇：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前兩種適合沒有容易氧化的氨基酸，例如Trp、Cys、Met。第三種適合幾乎所有的序列。）
6、純化凍干：使用液相色譜純化，收集目標(biāo)峰液體，進(jìn)行凍干，獲得蓬松的粉末狀固體多肽。不過這時要取小樣復(fù)測下純度 是否目標(biāo)純度。
7、最后總結(jié)：
杭州專肽生物技術(shù)有限公司（ALLPEPTIDE http://tsjxdd.com）主營定制多肽合成業(yè)務(wù)，提供各類長肽，短肽，環(huán)肽，提供各類修飾肽，如：熒光標(biāo)記修飾（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基團修飾肽（疊氮、炔基、DBCO、DOTA、NOTA等），同位素標(biāo)記肽（N15、C13），訂書肽（Stapled Peptide）,脂肪酸修飾肽（Pal、Myr、Ste），磷酸化修飾肽（P-Ser、P-Thr、P-Tyr），環(huán)肽（酰胺鍵環(huán)肽、一對或者多對二硫鍵環(huán)），生物素標(biāo)記肽，PEG修飾肽，甲基化修飾肽等。
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